The Ultimate Guide To Identifying Osteoclasts
1 / 5 2 / 5 3 / 5 4 / 5 5 / 5 ❮ ❯ · a form of ai was developed and trained to classify four different cell types, with a particular focus on identifying, counting, and determining the maturity of osteoclasts. Tibiae from wistar rats were either enzymatically stained for trap or immunostained for cathepsin k to identify … · to accelerate the process and reduce the workload for counting the number of osteoclasts, we developed oc_finder, a fully automated system for identifying osteoclasts in … These chemical traces are called bone … We present the first detailed step-by-step protocol to determine osteoclast-covered bone surfaces (oc. s/bs) using ai and compare the results directly with those obtained by the conventional … · the software described is intended to be used for image analyzation during cell culture of monocytes and osteoclasts for identification of osteoclastic maturation. During bone turnover, the specialist cells that break down old bone (osteoclasts), and lay down new bone (osteoblasts), leave chemical traces behind. · we present a detailed step-by-step guide for the ai-based method. · osteoclasts use their ruffled borders (with villous extensions) to bind to matrix adhesion proteins, produce resorption pits / bays (shallow concavities) called howship lacunae Functioning as vital cells in the bone matrix that maintains adequate bone mineral density through stimulating bone turnover and maintaining plasma calcium levels, osteoclasts are multinucleate … We cover in detail both traditional and more modern methods of assessing osteoclast formation and function in vitro including advances in image acquisition and automated analyses. Osteoclasts are not derived from osteoprogenitor cells. They are derived from blood monocytes/macrophages which are derived from haemopeoitic cells in the bone marrow. ...
